[1]张贺楠#,齐 岩#,史伟伟,等.血管瘤病变型J亚群禽白血病病毒E元件缺失突变体的构建[J].中国预防兽医学报,2010,(02):94-97.
 ZHANG He-nan#,QI Yan#,SHI Wei-wei,et al.Construction of an infectious clone of subgroup J Avian leukosis virus with E element deletion[J].Chinese journal of preventive veterinary medicine,2010,(02):94-97.
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血管瘤病变型J亚群禽白血病病毒E元件缺失突变体的构建
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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2010年02期
页码:
94-97
栏目:
病原生物学
出版日期:
2010-02-01

文章信息/Info

Title:
Construction of an infectious clone of subgroup J Avian leukosis virus with E element deletion
文章编号:
1008-0589(2010)02-0094-04
作者:
张贺楠12#齐 岩123#史伟伟12梁艺瑜12刘洪波12张小桃1曹伟胜12*廖 明12*
1. 华南农业大学 兽医学院/农业部动物疫病防控重点开放实验室,广东 广州510642;
2. 农业部动物疫病防控重点开放实验室,广东 广州 510642;
3. 中国牧工商(集团)总公司,北京 100070
Author(s):

ZHANG He-nan12# QI Yan123# SHI Wei-wei12 LIANG Yi-yu12 LIU Hong-bo12 ZHANG Xiao-tao12 CAO Wei-sheng12* LIAO Ming12*

1. The South China Agricultural University, Key Laboratory of Animal Disease Control and Prevention , Ministry of Agriculture; College of Veterinary Medicine, Guangzhou 510642, China;
2. Key Laboratory of Animal Disease Control and Prevention, Ministry of Agriculture, Guangzhou 510642, China;
3. China Animal Husbandery Group, Beijing 100070, China
关键词:
J亚群禽白血病病毒血管瘤E元件感染性克隆
Keywords:
subgroup J Avian leukosis virus Hemangioma E element infectious clone
分类号:
S852.65
文献标志码:
A
摘要:
为构建血管瘤病变型J亚群禽白血病病毒E元件缺失突变体病毒,本研究利用融合PCR方法将E元件缺失,获得含有缺失性突变体病毒前病毒全基因组的重组质粒pSCAU-HN-△E。将该重组质粒纯化后转染DF-1细胞,并连续传代,最终获得缺失病毒rSCAU-HN-△E。该毒株感染的DF-1细胞可被ALV-J特异性单克隆抗体JE9所识别,并且体外增殖性能稳定。该缺失性病毒的获得为E元件功能的研究奠定了基础。
Abstract:
The Subgroup J avian leukosis virus (ALV-J) strain SCAU-HN06 isolated from commercial layer hens with spontaneous hemangiomas has a complete "E" element in 3’UTR. In order to rescue the E element-deleted virus, we constructed the recombinant plasmid pSCAU-HN-△E that contained the whole ALV-J genome but with E element deleted. The plasmid was transfected into DF-1 cells and the rescued virus was identified by indirect fluorescence antibody test with ALV-J specific monoclonal antibody JE9. The recombinant virus was stable in cell culture during nine passages and had a titre up to 103.6 TCID50/ 0.2 mL.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2009-11-10
基金项目:国家自然科学基金(30771612);NSFC-广东联合基金(U0831002);广东省自然科学基金(8151064201000065);广东省科技计划项目(20090204)
作者简介:张贺楠(1980-),女,黑龙江齐齐哈尔人,博士研究生,从事动物病毒分子生物学研究;
齐 岩(1980-),男,黑龙江齐齐哈尔人,博士研究生,从事动物病毒分子生物学研究.
更新日期/Last Update: 2010-05-14