[1]刘超男,高玉龙,高宏雷,等.J亚群与E亚群禽白血病自然重组病毒的全基因组序列分析[J].中国预防兽医学报,2009,(12):978-981.
 LIU Chao-nan,GAO Yu-long,GAO Hong-lei,et al.Molecular characterization of a natural recombinant subgroup J avian leukosis virus with subgroup E partial env gene[J].Chinese journal of preventive veterinary medicine,2009,(12):978-981.
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J亚群与E亚群禽白血病自然重组病毒的全基因组序列分析
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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2009年12期
页码:
978-981
栏目:
病毒及分子生物学
出版日期:
2009-12-15

文章信息/Info

Title:
Molecular characterization of a natural recombinant subgroup J avian leukosis virus with subgroup E partial env gene
文章编号:
1008-0589(2009)12-0978-04
作者:
刘超男12高玉龙1高宏雷1祁小乐1景 龙2潘 伟1王笑梅1*
1. 中国农业科学院 哈尔滨兽医研究所,黑龙江 哈尔滨150001;
2. 东北农业大学,黑龙江 哈尔滨 150030
Author(s):
LIU Chao-nan12 GAO Yu-long1 GAO Hong-lei1 QI Xiao-le1 JING Long2 PAN Wei1 WANG Xiao-mei1*
1. Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China;
2. Northeast Agricultural University, Harbin 150030, China
关键词:
J亚群禽白血病病毒血管瘤全基因序列重组
Keywords:
avian leukosis virus subgroup J hemangioma whole genome sequence recombinant
分类号:
S852.65
文献标志码:
A
摘要:
为了解我国东北地区部分养鸡场禽白血病病毒(ALV)的基因组序列特征及其变异情况,本研究从具有典型血管瘤病变的禽白血病发病鸡中分离到一株J亚群ALV (ALV-J)命名为JL0901,并进行了全基因测序。将该序列与已发表的ALV-J毒株序列进行比较研究,结果表明JL0901基因组的gag和pol基因相对保守,而env基因和3’端非编码区(3’UTR)的变异较大。对JL0901的env基因核苷酸序列进一步分析发现,在其gp85基因和gp37基因交界位置发生J亚群和E亚群ALV重组现象。本研究证实国内鸡群中存在J亚群和其他亚群ALV的自然重组现象,并表明国内ALV已出现新的变异趋势。
Abstract:
An avian leukosis disease virus strain named JL0901 was isolated from chicken with typical hemangioma symptom in Northeast region of China. The genome of the virus strain was sequenced and compared with the reported strains both domestically and overseas. The results showed that the gag and pol genes of the ALV-J strains were relatively conservative, as compared to more variable env gene and 3’UTR. Remarkably, the env gene sequence of JL0901 was shown to be a ALV-J/E natural recombinant containing 240 bp subgroup E sequence in the borderline of gp85 (SU) and gp37 (TM) gene. This was the first report that the avian leukosis virus subgroup J partly recombined with subgroup E env gene at this site in natural infection. Our studies suggest that ALV-J continue to evolve by recombination, generating new viruses with different pathological properties in China.

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备注/Memo

备注/Memo:

收稿日期:2009-10-12
基金项目:现代农业肉鸡产业技术体系建设(nycytx-42-G3-01);国家重点实验室开放基金(SKLVBD200901)
作者简介:刘超男(1978-),男,黑龙江桦南人,讲师,博士,主要从事禽病免疫病理研究.



更新日期/Last Update: 2010-06-12