[1]宋红梅,杨 涛,马健男,等.蓝舌病病毒VP7基因的原核表达[J].中国预防兽医学报,2009,(12):925-928.
 SONG Hong-mei,YANG Tao,MA Jian-nan,et al.Prokaryotic expression of Bluetongue virus VP7 gene [J].Chinese journal of preventive veterinary medicine,2009,(12):925-928.
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蓝舌病病毒VP7基因的原核表达
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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2009年12期
页码:
925-928
栏目:
病毒及其病原生物学
出版日期:
2009-12-15

文章信息/Info

Title:

Prokaryotic expression of Bluetongue virus VP7 gene


 

作者:
宋红梅12杨 涛2马健男2王 群2张维军2徐青元2杨增岐1吴东来2*
(1. 西北农林科技大学 动物医学院,陕西 杨凌 712100;2. 中国农业科学院哈尔滨兽医研究所
兽医生物技术国家重点实验室/农业部兽医公共卫生重点开放实验室,黑龙江 哈尔滨 150001)
Author(s):
SONG Hong-mei12 YANG Tao2 MA Jian-nan2 WANG Qun2 ZHANG Wei-jun2 XU Qing-yuan2 YANG Zeng-qi1 WU Dong-lai2*
(1. College of Veterinary Medicine, Northwest Sci-Tech University of Agriculture and Forestry, Yangling 712100, China; 2. The Key Laboratory of Veterinary Public Health, Ministry of Agriculture/State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China)
关键词:
蓝舌病病毒VP7基因原核表达
Keywords:
 bluetongue virus VP7 gene prokaryotic expression
文献标志码:
A
摘要:
为获得蓝舌病病毒(BTV) VP7基因的原核表达蛋白,本实验采用RT-PCR方法扩增出了血清1型BTV的VP7基因,并克隆到原核表达载体pMAL-c2X中,构建了重组质粒pMAL-VP7。将重组质粒转化TB1感受态细胞,以0.5 mmol/L的IPTG进行诱导表达,SDS-PAGE电泳结果显示融合蛋白MBP-VP7以可溶形式存在,分子质量约为90 ku。以纯化的重组蛋白作为包被抗原,初步建立了检测BTV血清抗体的间接ELISA诊断方法,为今后进一步开展BTV诊断研究奠定了基础。
Abstract:
In this study, the VP7 gene of serotype 1 bluetongue virus was amplified by RT-PCR and cloned into prokaryotic expression vector pMAL-c2X. The recombinant plasmid was transformed into E. coli TB1 cells and fusion protein (MBP-VP7) produced after induced with 0.5 mmol/L IPTG. The expressed protein was about 90 ku and existed in soluble form. Indirect ELISA was established using purified MBP-VP7 protein as antigen.

参考文献/References:

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[9]Oldfield S, Adachi A, Urakawa T, et a1. Purification and characterization of the major group-specific core antigen VP7 of bluetongue virus synthesized by a recombinant baculovirus[J]. J Gen Virol, 1990, 71: 2649-2656.

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备注/Memo

备注/Memo:
收稿日期:2009-04-10
基金项目:国家863项目(No.2007AA02Z406)
作者简介:宋红梅(1983-),女,山东烟台人,硕士研究生,主要从事分子病毒学和分子免疫学研究.
*通信作者:E-mail: dlwu@hvri.ac.cn
更新日期/Last Update: 2010-01-06