[1]姜艳平,葛俊伟,汪 淼,等.猪流行性腹泻病毒重组核蛋白作为检测抗原的初步应用[J].中国预防兽医学报,2008,(08):587-591.
 JIANG Yan-ping,GE Jun-wei,WANG Miao,et al.Preliminary application of the recombinant nucleocapsid protein of porcine epidemic diarrhea virus LJB/03 as detecting antigen in diagnostic assays[J].Chinese journal of preventive veterinary medicine,2008,(08):587-591.
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猪流行性腹泻病毒重组核蛋白作为检测抗原的初步应用
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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2008年08期
页码:
587-591
栏目:
病原生物学
出版日期:
2008-08-01

文章信息/Info

Title:
Preliminary application of the recombinant nucleocapsid protein of porcine epidemic diarrhea virus LJB/03 as detecting antigen in diagnostic assays
文章编号:
1008-0589(2008)08-0587-05
作者:
姜艳平葛俊伟汪 淼刘兆磊裴敦国韩梓峰李一经*
东北农业大学 动物医学院,黑龙江 哈尔滨 150030
Author(s):
JIANG Yan-ping GE Jun-wei WANG Miao LIU Zhao-lei PEI Dun-guo HAN Zi-feng LI Yi-jing*
College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China
关键词:
猪流行性腹泻病毒N蛋白诊断抗原
Keywords:
PEDV N protein detective antigen
分类号:
S852.659
文献标志码:
A
摘要:
为确定猪流行性腹泻病毒(PEDV)重组N蛋白作为检测抗原的可应用性,将N基因克隆至pProHTa载体,构建重组表达载体,而后转化大肠杆菌BL21感受态细胞并诱导表达。将表达的可溶性融合蛋白经Ni+亲和层析柱纯化,并对其进行Western blot、dot-ELISA、间接ELISA等免疫学实验分析。结果表明,重组N蛋白具有良好的抗原性和较高的敏感性,与其他腹泻病病毒的抗血清无交叉反应。因此,猪流行性腹泻病毒重组N蛋白作为检测抗原在监测病毒感染及评价疫苗免疫效果方面具有较高的应用价值,是代替全病毒作为检测抗原的良好选择。
Abstract:
The N gene of porcine epidemic diarrhea virus (PEDV) was cloned into the plasmid pProHTa to construct the recombinant expression vector. Recombinant N protein was expressed in E.coli BL21 by IPTG induction. The soluble His-tag- fused N protein was purified through Ni+ chromatography column and its antigenicity was validated by Western blot, dot-ELISA and indirect ELISA. The results showed that recombinant protein retained strong antigenicity of the native N protein and was highly specific. It is a valuable antigen alternative for diagnosis of virus infection and evaluation to vaccine efficiency.

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备注/Memo

备注/Memo:
收稿日期:2008-01-23
基金项目:黑龙江十一五科技攻关项目(GA06B202-4)
作者简介:姜艳平(1981~),女,黑龙江肇东人,硕士,主要从事微生物与分子生物学研究.
更新日期/Last Update: 2010-12-15