[1]韩庆功,乔传玲*,杨焕良,等.猪源H9N2亚型流感病毒HA基因的序列测定及真核表达[J].中国预防兽医学报,2007,(12):916-919.
 HAN Qing-gong,QIAO Chuan-ling*,YANG Huan-liang,et al.Sequencing and eukaryotic expression of HA gene of H9N2 subtype influenza virus from swine[J].Chinese journal of preventive veterinary medicine,2007,(12):916-919.
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猪源H9N2亚型流感病毒HA基因的序列测定及真核表达
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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2007年12期
页码:
916-919
栏目:
病原生物学
出版日期:
2007-12-01

文章信息/Info

Title:
Sequencing and eukaryotic expression of HA gene of H9N2 subtype influenza virus from swine
文章编号:
1008-0589(2007)12-0916-04
作者:
韩庆功12乔传玲1*杨焕良1陈 艳1辛晓光1丁选亚1陈化兰1*
1. 中国农业科学院哈尔滨兽医研究所 兽医生物技术国家重点实验室/农业部动物流感重点开放实验室,黑龙江 哈尔滨 150001;
2. 河南科技学院 动物科学学院,河南 新乡 453003
Author(s):
HAN Qing-gong12 QIAO Chuan-ling1* YANG Huan-liang1 CHEN Yan1 XIN Xiao-guang1 DING Xuan-ya1 CHEN Hua-lan1*
1. Animal Influenza Laboratory of Ministry of Agriculture, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, China;
2. College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang 453003, China
关键词:
猪流感病毒HA基因序列测定真核表达
Keywords:
swine influenza virus HA gene sequencing eukaryotic expression
分类号:
S852.659.5
文献标志码:
A
摘要:
利用RT-PCR扩增猪流感病毒A/Swine/Zhejiang/7/2004(H9N2)(SW/ZhJ7/04)HA基因,测序后将其克隆到真核表达载体pCAGGS上,经酶切分析、PCR鉴定和测序验证,得到重组表达质粒pCAGGS-HA,之后将其转染293T细胞,48 h后收集转染细胞样品,进行SDS-PAGE分析,结果显示HA蛋白获得了表达。经Western blot检测,结果表明pCAGGS-HA能够在体外瞬时表达具有免疫学活性的HA蛋白,通过免疫小鼠,对其免疫效果进行了初步研究。
Abstract:
HA gene of SW/ZhJ7/04 was amplified by RT-PCR, sequenced, and cloned into recombinant plasmid pCAGGS-HA. The 293T cells monolayer were transfected with plasmid pCAGGS-HA. expression of the HA protein was detected by SDS-PAGE 48 h after transfection and its immunogenic activity was confirmed by Western blot assay. The immunogenicity of the plasmid pCAGGS-HA was evaluated in mice.

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备注/Memo

备注/Memo:
收稿日期:2007-06-04
基金项目:国家科技攻关计划项目(2004BA519A55)
作者简介:韩庆功(1976~),男,河南汝州人,硕士研究生,主要从事猪流感病毒的研究.
*通讯作者:E-mail:hlchen@hvri.ac.cnqcl@hvri.ac.cn
更新日期/Last Update: 2011-01-06