[1]孙东波,冯 力*,时洪艳,等.猪流行性腹泻病毒S蛋白中和表位区单克隆抗体的制备与鉴定[J].中国预防兽医学报,2007,(11):887-890.
 SUN Dong-bo,FENG Li*,SHI Hong-yan,et al.Preparation and characterization of the monoclonal antibodies against neutralizing epitope region of PEDV S protein[J].Chinese journal of preventive veterinary medicine,2007,(11):887-890.
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猪流行性腹泻病毒S蛋白中和表位区单克隆抗体的制备与鉴定
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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2007年11期
页码:
887-890
栏目:
免疫学
出版日期:
2007-11-01

文章信息/Info

Title:
Preparation and characterization of the monoclonal antibodies against neutralizing epitope region of PEDV S protein
文章编号:
1008-0589(2007)11-0887-04
作者:
孙东波冯 力*时洪艳陈建飞崔晓辰佟有恩
中国农业科学院哈尔滨兽医研究所 兽医生物技术国家重点实验室/猪传染病研究室,黑龙江 哈尔滨 150001
Author(s):
SUN Dong-bo FENG Li* SHI Hong-yan CHEN Jian-fei CUI Xiao-chen TONG You-en
Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, China
关键词:
猪流行性腹泻病毒S蛋白中和表位区单克隆抗体
Keywords:
porcine epidemic diarrhea virus S protein neutralizing epitope region McAbs
分类号:
S852.659.6
文献标志码:
A
摘要:
以纯化的猪流行性腹泻病毒(Porcine epidemic diarrhea virus,PEDV)S蛋白中和表位(S1D)重组蛋白为抗原免疫BALB/c小鼠,利用淋巴细胞杂交瘤技术获得了6株稳定分泌抗S1D区特异性的单克隆抗体细胞株,分别命名为2C4、3G3、5F8、3G5、6E6和3C3。6株杂交瘤细胞诱生小鼠产生的腹水抗体效价分别为1∶51 200、1∶6 400、1∶12 800、1∶6 400、1∶51 200和1∶25 600。免疫球蛋白类型均为IgG1型,轻链均为κ链。Western blot试验结果显示,6株单克隆抗体均能特异性地识别天然PEDV中的S蛋白。
Abstract:
The purified neutralizing epitope region of PEDV S protein, S1D, was used to immunize BALB/c mice for cell fusion. Six hybridoma cell lines secreting monoclonal antibodies (McAbs) against S1D were established and designated as 2C4, 3G3, 5F8, 3G5, 6E6 and 3C3, respectively. All six McAbs were IgG1 subtype and had κ chain. The McAbs had ascetic titers of 1∶51 200, 1∶6 400, 1∶12 800, 1∶6 400, 1∶51 200 and 1∶25 600 respectively. Western blot confirmed that all McAbs were able to reacted with native S protein of PEDV.

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备注/Memo

备注/Memo:
收稿日期:2007-03-12
作者简介:孙东波(1978~),男,黑龙江密山人,博士研究生,主要从事动物病毒分子生物学研究.
*通讯作者:E-mail:fl@hvri.ac.cn
更新日期/Last Update: 2011-01-17