[1]张辉,陈创夫*,王远志,等.羊种布鲁氏菌的分离鉴定及其ugpB 基因的原核表达[J].中国预防兽医学报,2009,(05):356-360.
 ZHANG Hui,CHEN Chuang-fu*,WANG Yuan-zhi,et al.Isolation and identification of Brucella melitensis and prokaryotic expression of its ugpB gene[J].Chinese journal of preventive veterinary medicine,2009,(05):356-360.
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羊种布鲁氏菌的分离鉴定及其ugpB 基因的原核表达
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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2009年05期
页码:
356-360
栏目:
病毒及分子生物学
出版日期:
2009-05-15

文章信息/Info

Title:
Isolation and identification of Brucella melitensis and prokaryotic expression of its ugpB gene
作者:
张辉12陈创夫12*王远志3盛金良2任艳2郭飞2
(1. 新疆地方与民族高发病教育部重点实验室,新疆石河子832003;
2. 石河子大学动物科技学院,新疆石河子832003;3. 石河子大学医学院,新疆石河子832003)
Author(s):
ZHANG Hui12 CHEN Chuang-fu12* WANG Yuan-zhi3 SHENG Jin-liang2 REN Yan2 GUO Fei2
(1. Key Laboratory of Xinjiang Endemic and Ethnic Disease, Shihezi 832003, China; 2. College of Animal Science and Technology of Shihezi University, Shihezi 832003, China; 3. College of Medicine of Shihezi University, Shihezi 832003, China)
关键词:
羊种布鲁氏菌分离鉴定ugpB 基因蛋白纯化
Keywords:
Brucella melitensis isolation identification ugpB gene protein purification
文献标志码:
A
摘要:
对新疆某羊场流产胎儿进行布鲁氏菌病原分离、培养,采用细菌群体形态观察、PCR、生化试验进行鉴定,结果分离得到了羊种布鲁氏菌生物3 型,命名为027 株。应用常规分子生物学方法克隆羊种布鲁氏菌生物3 型027 株的ugpB 基因,构建原核表达载体pET-ugpB,转化BL21 (DE3),IPTG 诱导表达重组蛋白UgpB,进行SDS-PAGE 和western blot 分析,结果表明ugpB 融合基因在大肠杆菌中得到了表达;采用Ni-NTA Agarose 试剂盒进行蛋白纯化,获得了纯化的融合蛋白。
Abstract:
A  Brucella  isolate was isolated from aborted fetuses at a sheep farm in Xinjiang and characterized by PCR, biochemical assays. The result showed that the isolate belonged to Brucella melitensis biovar 3, and named Brucella melitensis 027. The ugpB gene of 027 strain was cloned into prokaryotic expression vector pET-32a (+) and transformed into E.coli BL21 (DE3) cells. Expression of the fusion protein ugpB was induced by IPTG and confirmed by SDS-PAGE and western blot.

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更新日期/Last Update: 2009-06-09