[1]王月丽,易继海,王 震,等.布鲁菌对不同分化类型巨噬细胞JAK-STAT6信号通路的影响 [J].中国预防兽医学报,2017,(10):836-841.[doi:10.3969/j.issn.1008-0425.201703049]
 WANG Yue-li,YI Ji-hai,WANG Zhen,et al.Effects of Brucella on JAK-STAT6 signaling pathway in different differentiation types of macrophages[J].Chinese journal of preventive veterinary medicine,2017,(10):836-841.[doi:10.3969/j.issn.1008-0425.201703049]
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布鲁菌对不同分化类型巨噬细胞JAK-STAT6信号通路的影响

 

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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2017年10
页码:
836-841
栏目:
出版日期:
2017-10-15

文章信息/Info

Title:
Effects of Brucella on JAK-STAT6 signaling pathway in different differentiation types of macrophages
文章编号:
1008-0589(2017)10-0836-06
作者:
 王月丽1 易继海1 王 震1唐 燕 1童志霞1徐锦凤2张 辉1*陈创夫1*
 (1. 石河子大学 动物科技学院,新疆 石河子 832000;2. 石河子大学 生命科学学院,新疆 石河子 832000)
Author(s):
 

WANG Yue-li1? YI Ji-hai1? WANG Zhen1 TANG Yan1 TONG Zhi-xia1 XU Jin-feng2ZHANG Hui1* CHEN Chuang-fu1*

 (1.College of Animal Science and Technology, Shihezi University, Shihezi 832000, China;
2. College of Life Sciences, Shihezi University, Shihezi 832000, China)
关键词:
布鲁菌巨噬细胞极化JAK-STAT6信号通路
分类号:
S852.61
DOI:
10.3969/j.issn.1008-0425.201703049
文献标志码:
A
摘要:
为研究布鲁菌侵染巨噬细胞过程中,对后者不同分化类型细胞中JAK-STAT6信号通路和细胞因子表达变化的影响,本研究利用小鼠巨噬细胞系RAW264.7,经40 ng/mL的IL-4体外作用24 h将其诱导为M2型巨噬细胞,采用细胞免疫荧光方法,检测M2巨噬细胞极化状态;通过荧光定量PCR (qRT-PCR)方法,检测布鲁菌疫苗株M5侵染不同类型(M0和M2型)巨噬细胞中JAK1、STAT6 基因mRNA转录情况;采用ELISA方法检测M5侵染不同类型巨噬细胞上清中IL-10、INF-γ的表达情况。细胞免疫荧光检测结果显示,IL-4诱导24 h后,巨噬细胞表面标志CD206荧光表达量超过90 %,表明巨噬细胞极化为M2型; qRT-PCR结果显示,M5侵染M0型巨噬细胞后能够引起JAK1、STAT6 mRNA转录水平持续降低,在24 h和48 h时其转录水平最低,二者差异极显著(p<0.01);而M5侵染M2型巨噬细胞后,与未极化组(M0型)相比,JAK1、STAT6的mRNA转录水平增高,同时存在时间差异性,侵染8 h时,二者mRNA转录水平最高,差异极显著(p<0.01)。ELISA结果显示,M5侵染巨噬细胞后,极化组(M2型)与未极化组(M0型)巨噬细胞相比,INF-γ的表达量差异不显著(p>0.05),而IL-10的表达量上调且在侵染48 h达到最大值,差异显著(p<0.05)。上述结果表明,在IL-4的诱导下巨噬细胞可以从未极化的M0型极化为M2型;布鲁菌M5侵染M0型巨噬细胞中JAK1、STAT6的转录水平下调,但上调M2型巨噬细胞JAK1、STAT6的转录水平并促进下游细胞因子IL-10的分泌。本研究为探究布鲁菌引起持续感染的机制研究奠定了基础,同时为布鲁菌病药物靶标开拓了新的研究方向。
  
Abstract:
To investigate the effect of Brucella infection on the changes of JAK-STAT6 signaling pathway and cytokine expression in different differentiated cell types of macrophages, murine macrophage lineage RAW264.7 was induced to M2 type

参考文献/References:

[1]董炳梅,王金良,唐娜,等. 布鲁菌的致病机制与细胞免疫机制研究进展[J]. 中国人兽共患病学报,2012,28(6):635-639.
[2]权伍荣,杨咏洁. 布鲁氏菌逃逸宿主的抗感染免疫机制[J].微生物学报,2016,(05):747-752.
[3]Gordon S, Martinez F O. Alternative activation of macrophages: mechanism and functions [J]. Immunity, 2010, 32(5): 593-604.
[4]Souza-Neto J A, Sim S, Dimopoulos G. An evolutionary conserved function of the JAK/STAT pathway in anti-dengue defense [J]. PNAS USA, 2009, 106(42): 17841-17846.
[5]Liao Xu-dong, Sharma N, Kapadia F, et al. Krüppel-like factor 4 regulates macrophage polarization [J]. J Clin Invest, 2011, 121(7): 2736-2749.
[6]Van Dyken S J, Locksley R M. Interleukin-4-andinterleukin-13- mediated alternatively activated macrophages: roles in homeostasis and disease [J]. Annu Rev Immunol, 2013, 31: 317-343.
[7]Benoit M, Desnues B, Mege J. Macrophage polarization in bacterial infections [J]. Immunol, 2008, 181: 3733-3739.
[8] Eisele N A, Ruby T, Jacobson A, et al. Salmonella require the fatty acid regulator PPARδ for the establishment of a metabolic environment essential for long-term persistence [J]. Cell Host Microbe, 2013, 14: 171-182.
[9]Novak M L, Koh T J. Macrophage phenotypes during tissue repair [J]. Leukoc Biol, 2013, 93(6): 875-881.
[10]宋舟,张立艳,董海兵,等. JAK-STAT信号通路研究进展[J]. 中国畜牧兽医,2012,(06):128-132.
[11]Shirey K A, Cole L E, Keegan A D. Francisella tularensis live vaccine strain induces macrophage alternative activation as a survival mechanism [J]. J Immunol, 2008, 181(6), 4159-4167.
[12]Durward M, Radhakrishnan G, Harms J, et al. Active evasion of CTL mediated killing and low quality responding CD8+T cells contribute to persistence of brucellosis [J]. PLoS One, 2012, 7(4): e34925.
[13]Skendros P, Pappas G, Boura P. Cell-mediated immunity in human brucellosis [J]. Microbes Infect, 2011, 13(2): 134-142.
                           (本文编辑:李 娜)

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更新日期/Last Update: 2017-11-06