[1]张会雷,刘任强,张敏敏,等.非洲猪瘟病毒结构蛋白基因重组痘苗病毒的构建及鉴定[J].中国预防兽医学报,2017,(10):789-793.[doi:10.3969/j.issn.1008-0425.201703042]
 ZHANG Hui-lei,LIU Ren-qiang,ZHANG Min-min,et al.Construction of recombinant vaccinia virus expressing structural protein of African swine fever virus[J].Chinese journal of preventive veterinary medicine,2017,(10):789-793.[doi:10.3969/j.issn.1008-0425.201703042]
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非洲猪瘟病毒结构蛋白基因重组痘苗病毒的构建及鉴定()
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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2017年10
页码:
789-793
栏目:
病原生物学
出版日期:
2017-10-15

文章信息/Info

Title:
Construction of recombinant vaccinia virus expressing structural protein of African swine fever virus
文章编号:
1008-0589(2017)10-0789-05
作者:
 

张会雷刘任强张敏敏王喜军葛金英温志远步志高*

 (中国农业科学院哈尔滨兽医研究所 兽医生物技术国家重点实验室,黑龙江 哈尔滨 150069)
Author(s):
 

ZHANG Hui-lei LIU Ren-qiang ZHANG Min-min WANG Xi-jun GE Jin-yingWEN Zhi-yuan BU Zhi-gao*

 (State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute,
Chinese Academy of Agricultural Sciences, Harbin 150069, China)
关键词:
非洲猪瘟病毒重组痘苗病毒
Keywords:
African swine fever virus  recombinant vaccinia virus
分类号:
S852.65
DOI:
10.3969/j.issn.1008-0425.201703042
文献标志码:
A
摘要:
非洲猪瘟(ASF)是由非洲猪瘟病毒(ASFV)引起的一种猪的传染病,发病率和死亡率几乎达100 %,是尚未传入我国的重要的猪烈性病。为研制安全有效的ASF储备疫苗,本研究利用本实验室改造的痘苗病毒(VV),将ASFV的结构蛋白P72和P54或CD2v和P30的基因分别插入到VV基因组中,构建了同时表达P72和P54的重组VV (rVV-P72-P54)以及同时表达CD2v和P30的重组VV (rVV-CD2v-P30)。Western blot和间接免疫荧光试验表明外源基因在重组病毒感染的细胞中均能够正确表达。这两株重组病毒在体外细胞培养中的增殖效率与亲本病毒无显著差异。该研究为进一步的动物免疫实验奠定了基础。
 
Abstract:
African swine fever is a fatal disease of pigs caused by African swine fever virus with morbidity and mortality rates approaching 100 percent, which has not been reported in China as a potent disease of great importance. To develop safe, effective vaccines against ASF, two recombinant vaccinia viruses (VV) were generated based on modified VV, and co-expressed P72 and P54 or co-expressed CD2v and P30. The result of western blot and immunofluorescence assay indicated that the four proteins in recombinant viruses were expressed effectively in infected cells. There was no significant difference between the two recombinant viruses and the original strain in efficiency of virus proliferation and the construction of two recombinant viruses lay the foundation for  further study on animal immune experiment.
  

参考文献/References:

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                                          (本文编辑:李 娜)

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更新日期/Last Update: 2017-11-06