[1]卜德新,张伟东,卜春玲,等.两株H1亚型猪流感病毒对猪的致病能力分析[J].中国预防兽医学报,2017,(10):784-788.[doi:10.3969/j.issn.1008-0425.201702029]
 BU De-xin,ZHANG Wei-dong,BU Chun-ling,et al.Two different strains of subtype H1 swine influenza viruses pathogenicity analysis to swine[J].Chinese journal of preventive veterinary medicine,2017,(10):784-788.[doi:10.3969/j.issn.1008-0425.201702029]
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两株H1亚型猪流感病毒对猪的致病能力分析()
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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2017年10
页码:
784-788
栏目:
病原生物学
出版日期:
2017-10-15

文章信息/Info

Title:
Two different strains of subtype H1 swine influenza viruses pathogenicity analysis to swine
文章编号:
1008-0589(2017)10-0784-05
作者:
 

卜德新张伟东卜春玲黄俊明蔡孟楷曹振鹏方 博付新亮亓文宝张桂红*

 (广东省动物源性人兽共患病预防与控制重点实验室 国家生猪种业工程技术研究中心/华南农业大学兽医学院,广东  广州  510642)
Author(s):
 

BU De-xin ZHANG Wei-dong BU Chun-ling HUANG Jun-ming CAI Meng-kai CAO Zhen-peng FANG Bo FU Xin-liang QI Wen-bao ZHANG Gui-hong*

 (1. College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China; 2. Animal Influenza Key Laboratory of the Ministry of Agriculture, State Key Laboratory of Veterinary Biotecnology, Harbin Veterinary Research Insitute,
Chinese Academy of Agricultural Sciences, Harbin 150069, China)
关键词:
H1亚型猪流感病毒病毒滴度致病性
Keywords:
 H1 subtype  swine influenza virus  virus titer  pathogenicity
分类号:
S852.65
DOI:
10.3969/j.issn.1008-0425.201702029
文献标志码:
A
摘要:
为研究H1亚型猪流感病毒分离株A/Swine/Guangdong/SS1/2012 (H1N1,SS1)和A/Swine/Guangdong/ YJ28/2014(H1N2,YJ28)的致病力,本研究对2株病毒基因组进行分析,并将2株病毒分别通过滴鼻途径感染仔猪后观察临床症状,在攻毒后4 d与7 d迫杀实验猪采集脏器样品,检测脏器病毒滴度,进行肺病理切片观察和免疫组化实验,比较2株猪流感病毒对猪的致病能力和排毒情况。遗传进化结果显示,SS1分离株8个节段均属于欧亚类禽分支,YJ28为欧亚类禽、pdm/09和人季节性流感的新型重组病毒。动物实验结果显示,两株猪流感病毒均能够在猪只上复制,临床症状均不明显。鼻拭子排毒检测结果显示,YJ28组猪排毒仅持续至感染后第6 d,但SS1组猪在整个实验周期均能检测到排毒。实验猪脏器病毒检测和病理切片结果显示,SS1组相比于YJ28组脏器病毒滴度更高,且造成更严重的肺部病理损伤。本实验分离株SS1相比YJ28在PB2基因出现D701N突变,这或许是SS1比YJ28具有更强致病能力和更长排毒周期的原因。本研究结果为H1N1和H1N2猪流感病毒对猪的致病能力和排毒情况提供实验依据。
  
Abstract:
To study the pathogenicity of swine influenza viruses A/Swine/Guangdong/SS1/2013 (H1N1, SS1) and A/Swine/Guangdong/YJ28/2014 (H1N2, YJ28), healthy piglets were inoculated with SS1 and YJ28, and observated clinical symptoms. At the 4th day and the 7th day after intranasal infection, organ samples were collected to measure the organ virus titer, the lung histopathological and immunohistochemical experiment were carride out. The genetic evolution results showed that the 8 segments of SS1 all belonged to the Eurasian Avian -like swine Influenza lineage (EA), and YJ28 was a novel triplereassortant from the EA, pdm/09 and Human seasonal lineage. The results of the animal experiments showed that the two swine influenza viruses could be replicated in pigs, but the clinical symptoms were not obvious. The kinetics of nasal excretion showed the pigs of YJ28 group detoxification only lasted to the 6th day after infection, but SS1 group could be detected detoxification during the whole experimental period. The experimental pig organ virus detection and pathological biopsy results showed that the pigs of SS1 group had a higher viral titer in organ than that of the pigs in YJ28 group, and SS1 could cause more severe pulmonary pathological damage. Compared with YJ28, the isolate SS1 had a D701N mutation in the PB2 gene, which could be the reason that SS1 had stronger pathogenic ability and longer detoxification cycle than YJ28. The results of this study could provide a experimental basis for research H1N1 and H1N2 swine influenza virus on the pathogenicity and  detoxification of swine.

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(本文编辑:彭永刚)

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更新日期/Last Update: 2017-11-06