[1]蔺利娟,王瑞琴,韩宗玺,等.鹌鹑β- 防御素10基因的克隆与表达及其体内分布的检测[J].中国预防兽医学报,2011,(07):552-556.
 LIN Li-juan,WANG Rui-qin,HAN Zong-xi,et al.Cloning and expression of quail avian beta-defensin 10 and the mRNA detection in quail tissues[J].Chinese journal of preventive veterinary medicine,2011,(07):552-556.
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鹌鹑β- 防御素10基因的克隆与表达及其体内分布的检测
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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2011年07期
页码:
552-556
栏目:
免疫学
出版日期:
2011-07-15

文章信息/Info

Title:
Cloning and expression of quail avian beta-defensin 10 and the mRNA detection in quail tissues
作者:
蔺利娟;王瑞琴;韩宗玺;邵昱昊;刘胜旺;程宝晶;孙黎;李子瑞;马得莹;
东北农业大学动物营养研究所;中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/禽传染病研究室;
Author(s):
LIN Li-juan12 WANG Rui-qin12 HAN Zong-xi2 SHAO Yu-hao2 LIU Sheng-wang2 CHENG Bao-jing1 SUN Li1 LI Zi-rui1 MA De-ying1
1. Institute of Animal Nutrition, Northeast Agricultural University, Harbin 150030, China; 2. Division of Avian Infectious Disease, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sci
关键词:
鹌鹑AvBD10重组蛋白抗菌活性组织分布
Keywords:
quail AvBD10 recombinant protein antimicrobial activity tissues distribution
摘要:
为测定鹌鹑β-防御素10(AvBD10)体外抗菌活性及检测其在鹌鹑脏器组织的分布,本研究应用RT-PCR方法从鹌鹑肺脏组织中扩增到鹌鹑AvBD10,经序列同源性分析,鹌鹑AvBD10与鸡AvBD10的氨基酸序列同源性最高(83.8%)。将该基因亚克隆到表达载体pGEX-6p-1中进行原核表达,SDS-PAGE电泳表明,鹌鹑AvBD10重组蛋白分子量约为31ku。该重组蛋白经纯化后测定其体外抗菌活性,结果显示,鹌鹑AvBD10重组蛋白具有广谱的抗菌活性。采用定量RT-PCR法检测到AvBD10在鹌鹑脏器组织中广泛表达。
Abstract:
To clone and express avian beta-defensin (AvBD10) gene in quail, the quail AvBD10 gene was amplified from lung of Chinese painted quail (Coturnix chinensis) by RT-PCR. Sequence analysis showed that the cDNA of quail AvBD10 consisted of 207 bp nucleotide, encoding a polypeptide of 68 amino acids. Homology analysis showed that quail AvBD10 shared 83.8% with chicken AvBD10. The cDNA of quail AvBD10 was sub-cloned into pGEX-6p-1 vector and expressed in E. coli BL21. SDS-PAGE analysis indicated that 31 ku of quail AvBD10 recombinant protein was highly expressed. The purified recombinant quail AvBD10 exhibit extensive antimicrobial activity against 10 different bacteria. Additionally, results of real-time RT-PCR detections showed that AvBD10 was widely expressed in various quail tissues

参考文献/References:

[1] 温娟. 鹌鹑大肠杆菌病的诊治[J]. 广西畜牧兽医. 2001年03期
[2] 王学新. 鹌鹑的饲养管理技术[J]. 养禽与禽病防治. 1996年12期
[3] 李树珩,刘书贤. 严防鹌鹑痘发生[J]. 畜牧兽医科技信息. 1996年06期
[4] 刘洪敏,李忠显,李处义. 鹌鹑大肠杆菌病的诊断与防治[J]. 中国家禽. 1997年04期
[5] 欧阳龙,刘洪敏,李忠显,陈哲. 鹌鹑大肠杆菌病的诊治[J]. 中国兽医杂志. 1998年10期
[6] 高云香. 鹌鹑新城疫的防制[J]. 河北畜牧兽医. 2001年09期
[7] 德贵. 鹌鹑新城疫防治技术[J]. 农村实用技术. 2004年06期
[8] 童建松. 鹌鹑种蛋的选择与贮运[J]. 农家顾问. 2005年10期
[9] 关梅. 如何使鹌鹑多产蛋[J]. 农村实用技术与信息. 2006年09期
[10] 邹铭之. 鹌鹑的主要器官数值测量分析[J]. 中国畜牧杂志. 1987年04期

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备注/Memo

备注/Memo:
基金:国家自然科学基金(30972110);;黑龙江省教育厅重点项目(1153LZ05)
更新日期/Last Update: 2011-09-29