[1]陈娜莎,华荣虹,闫丽萍,等.乙型脑炎病毒E蛋白抗原表位多肽序列鉴定及分析[J].中国预防兽医学报,2011,(07):541-545.
 CHEN Na-sha,HUA Rong-hong,YAN Li-ping,et al.Identification of an antigenic epitope on envelope glycoprotein of Japanese encephalitis virus[J].Chinese journal of preventive veterinary medicine,2011,(07):541-545.
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乙型脑炎病毒E蛋白抗原表位多肽序列鉴定及分析
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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2011年07期
页码:
541-545
栏目:
免疫学
出版日期:
2011-07-15

文章信息/Info

Title:
Identification of an antigenic epitope on envelope glycoprotein of Japanese encephalitis virus
作者:
陈娜莎;华荣虹;闫丽萍;赵付荣;王斌;杜鹃;王云峰;童光志;
中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室;中国农业科学院上海兽医研究所;新疆农业大学动物医学院;黑龙江大学生命科学与技术学院;
Author(s):
CHEN Na-sha1 HUA Rong-hong1 YAN Li-ping2 ZHAO Fu-rong3 WANG Bin1 DU Juan4 WANG Yun-feng1 TONG Guang-zhi2
1. State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China; 2. Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241
关键词:
乙型脑炎病毒囊膜糖蛋白(E蛋白)抗原表位
Keywords:
Japanese encephalitis virus envelope glycoprotein (E protein) antigenic epitope
摘要:
为了对流行性乙型脑炎病毒(JEV)E蛋白抗原表位E19进行核心序列定位,本研究设计了一系列编码相互部分重叠短肽核苷酸序列,原核融合表达后经westernblot分析,确定其抗原表位核心序列为150ENHGNYS156。该表位在乙型脑炎不同基因型的各种病毒株间为高度保守序列;根据JEVE19表位与同一血清群其他黄病毒之间的差异,在同源序列位置设计了系列突变短肽,融合表达后western blot分析的结果表明,其他黄病毒属病毒同源序列不能与抗JEV阳性血清反应。本实验结果表明JEVE蛋白抗原表位E19具有鉴别检测JEV与西尼罗病毒等其它黄病毒的意义。本实验为进一步研究E蛋白结构和功能以及建立乙型脑炎临床鉴别诊断方法奠定了分子生物学基础。
Abstract:
To identify the antigen epitope on envelope glycoprotein (gE) of Japanese encephalitis virus (JEV), a series partial overlopping oligo DNAs encoding short peptides were synthesized based on gE within 145GTTTSENHGNYSAQVG160 of E19 epitope region by our previous studies. The core amino sequence of 150ENHGNYS156 was identified by western blot with anti-JEV sera to these short peptides expressed in E. coli. The homologous analysis indicated that the amino acid sequences of the epitope were highly conserved among different JEV strains. Furthermore, another series of short peptides were designed at the position of homologous sequence according to the difference flavivirus strains. Western blot result showed that the homologous sequences of flavivirus strains had no cross-reaction with anti-JEV sera. The findings of the present studies would provided basis for further study of JEV gE and development of differential diagnostic techniques for Japanese encephalitis from other flavivirus infection

参考文献/References:

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备注/Memo

备注/Memo:
基金:国家自然科学基金(30700027);;公益性行业科研专项(200803015)
更新日期/Last Update: 2011-09-29