[1]杨舒心,雷连成,杜崇涛,等.胸膜肺炎放线杆菌菌影疫苗免疫仔猪前后差异表达基因的鉴定与分析[J].中国预防兽医学报,2011,(04):276-280.
 YANG Shu-xin,LEI Lian-cheng*,DU Chong-tao,et al.Identification and analysis of differential expression genes in peripheral blood lymphocytes from piglet immunized by bacterial ghost of Actinobacillus pleuropneumoniae[J].Chinese journal of preventive veterinary medicine,2011,(04):276-280.
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胸膜肺炎放线杆菌菌影疫苗免疫仔猪前后差异表达基因的鉴定与分析
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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2011年04期
页码:
276-280
栏目:
病原生物学
出版日期:
2011-04-15

文章信息/Info

Title:
Identification and analysis of differential expression genes in peripheral blood lymphocytes from piglet immunized by bacterial ghost of Actinobacillus pleuropneumoniae
作者:
杨舒心;雷连成;杜崇涛;王瑜;谢芳;韩文瑜;
吉林大学畜牧兽医学院;中国农业科学院哈尔滨兽医研究所;
Author(s):
YANG Shu-xin1 LEI Lian-cheng1* DU Chong-tao1 WANG Yu1 XIE Fang2 HAN Wen-yu1
1. College of Animal Science and Verterinery, Jilin University, Changchun 130062, China; 2. Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China
关键词:
胸膜肺炎放线杆菌外周血淋巴细胞代表性差异分析差异表达基因
Keywords:
Actinobacillus pleuropneumoniae peripheral blood lymphocyte representational difference analysis differential expression genes
摘要:
为获得胸膜肺炎放线杆菌(APP)菌影诱导的仔猪淋巴细胞差异表达基因,本研究应用代表性差异分析技术构建APP菌影免疫前后正、反两个外周血淋巴细胞cDNA差减文库,并对文库中的差异基因进行克隆、测序和生物信息学分析。试验结果表明,正向文库中获得11个表达丰度上调的基因,其中7个基因与已知基因具有相似性,4个为未知新基因,经进一步功能注解发现,正向文库功能基因包括免疫信号传导相关蛋白RhoE、防御相关蛋白糖基转移样酶-1、上皮膜蛋白2、白介素-17和肿瘤免疫相关的周期素依赖性蛋白激酶抑制因子3等,这些功能基因表达丰度升高,可能有助于机体建立抗APP的免疫应答。
Abstract:
To screen differential expression genes in peripheral blood lymphocytes induced by ghost of Actinobacillus pleuropneumoniae, the forward and reverse two subtractive cDNA libraries were constructed from the peripheral blood lymphocytes of piglet vaccinated by bacterial ghost of A. pleuropneumoniae using representational difference analysis technique. The analysis identified differentially expressed transcripts. The results indicated that genes related to immunization signal transduction, disease defence related protein, epithelial membrane protein and interleukin-17, tumor immunity related factors were up-regulated after vaccinated, which may increase the immunity response

参考文献/References:

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备注/Memo

备注/Memo:
基金:国家自然科学基金项目(31072133)
更新日期/Last Update: 2011-09-29