[1]梁立滨,赵东明,李俊平,等.H5N1亚型禽流感病毒感染小鼠肺组织蛋白质组分析[J].中国预防兽医学报,2011,(04):261-264.
 LIANG Li-bin,ZHAO Dong-ming,LI Jun-ping,et al.Proteomic analysis of lung tissues of mice infected by H5N1 avian influenza virus[J].Chinese journal of preventive veterinary medicine,2011,(04):261-264.
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H5N1亚型禽流感病毒感染小鼠肺组织蛋白质组分析
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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2011年04期
页码:
261-264
栏目:
病原生物学
出版日期:
2011-04-15

文章信息/Info

Title:
Proteomic analysis of lung tissues of mice infected by H5N1 avian influenza virus
作者:
梁立滨;赵东明;李俊平;李雁冰;施建忠;姜永萍;陈化兰;
中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/农业部动物流感开放实验室;
Author(s):
LIANG Li-bin ZHAO Dong-ming LI Jun-ping LI Yan-bing SHI Jian-zhong JIANG Yong-ping CHEN Hua-lan*
Animal Influenza Laboratory of the Ministry of Agricultural, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China
关键词:
H5N1亚型禽流感病毒双向凝胶电泳蛋白质组病原宿主相互作用
Keywords:
avian influenza H5N1 virus iwo-dimensional gel electrophoresis proteome pathogen-host interaction
摘要:
为鉴定禽流感病毒(AIV)感染状态下的差异表达蛋白,本研究将H5N1亚型AIV感染组与对照组小鼠肺组织蛋白样品分别进行双向电泳分离,并采用ImageMaster 2D Platinum 6.0软件进行分析。在AIV感染72h后,共有9个表达差异显著的蛋白点(ratio>2,p<0.05),而且该蛋白在感染组均呈上调表达。将差异蛋白进行串联质谱分析,并对其中7个蛋白进行鉴定,包括:interferon-induced protein with tetratricopeptide repeats 3(IFIT-3)、ATP依赖的干扰素反应蛋白1(ADIR1)、γ干扰素诱导的p47鸟苷三磷酸酶(IRG)、T细胞受体a TA27、气味受体S86、胞苷单磷酸激酶2(Cmpk2)和肌球蛋白。将前3个蛋白基因采用荧光定量PCR方法进行mRNA水平的验证,所获得的结果与双向电泳结果一致。本研究为在蛋白质组水平进一步分析H5N1亚型AIV与宿主相互作用奠定了基础。
Abstract:
In this study, the differentially expressed proteins were investigated in H5N1 avian influenza virus (AIV) infected mice by two dimensional gel electrophoresis (2-DE). The lung tissue lysis from the AIV infected or control groups were extracted at 72 hours post-inoculation and separated by 2-DE. Nine protein spots were found to have notably differential expression between the 2 groups (ratio>2, p<0.05), up-regulated in the AIV infected mice. Seven of them were identified by MALDI-TOF/TOF, including ATP-dependent interferon response protein1 (ADIR1), interferon-induced protein with tetratricopeptide repeats 3 (IFIT-3), T cell receptor alpha TA27, Interferon-gamma-inducible p47 GTPase (IRG), Cytidine monophosphate kinase 2 (Cmpk2 protein), odorant receptor S86, myosin light chain 1/3. Moreover, the transcriptional levels of 3 genes (ADIR, IFIT-3, and IRG) were verified by real-time PCR, which was completely consistent with the proteomic analysis result. Our data could provide an insight into the AIV-host interactions

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备注/Memo

备注/Memo:
基金:国家自然科学基金(30825032)
更新日期/Last Update: 2011-09-29