[1]李雪松,陈欣,符芳,等.副猪嗜血杆菌黑龙江株的分离与鉴定[J].中国预防兽医学报,2011,(03):236-238.
 LI Xue-song,CHEN Xin,FU Fang,et al.Isolation and identification of Haemophilus parasuis in Heilongjiang area[J].Chinese journal of preventive veterinary medicine,2011,(03):236-238.
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副猪嗜血杆菌黑龙江株的分离与鉴定
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《中国预防兽医学报》[ISSN:1008-0589/CN:23-1417/S]

卷:
期数:
2011年03期
页码:
236-238
栏目:
研究简报
出版日期:
2011-03-15

文章信息/Info

Title:
Isolation and identification of Haemophilus parasuis in Heilongjiang area
作者:
李雪松;陈欣;符芳;王伟;田华彬;郎越坤;徐春红;李曦;李春艳;
单位:东北农业大学;中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/猪传染病研究室;哈尔滨师范大学;
Author(s):
LI Xue-song12CHEN Xin2FU Fang2WANG Wei3TIAN Hua-bin2LANG Yue-kun2XU Chun-hong1LI Xi2*LI Chun-yan1*
1.Northeast Agricultural University,Harbin 150030,China;2.Division of Swine Infectious Diseases,State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001,China;3.Harbin Normal University,Harbin 150001,China
关键词:
副猪嗜血杆菌分离鉴定16S rRNA基因
Keywords:
Haemophilus parasuis isolation and identification 16S rRNA gene
摘要:
为确定黑龙江某猪场发病猪群的病原,本研究从疑似浆膜炎的病料中分离到一株革兰氏阴性细小杆菌,通过对其进行细菌形态学、培养特征、生化特性和16S rRNA基因PCR鉴定,结果表明该分离菌株为副猪嗜血杆菌(HLJ-1分离株)。16S rRNA基因序列分析结果显示:该分离菌株与参考株的基因同源性达99%;药敏试验结果表明HLJ-1分离株对头孢曲松、头孢噻肟等β-内酰胺类抗生素高度敏感;小白鼠致病性试验结果显示,该分离株具有较强致病性。
Abstract:
A small polymorphic gram negative bacterium was isolated from affected pig in Heilongjiang area.The bacterium was identified as Haemophilus parasuis(HLJ-1) by bacteria morphology,cultural character,physiological and biochemical characteristics and 16S rRNA gene PCR.Result revealed that 16S rRNA gene of HLJ-1 isolate shared the homology of 99% with reference strains.Drug sensitive tests showed that HLJ-1 was highly sensitive to β-lactam antibiotics,such as Ceftriaxone and Cefotaxime,and less sensitive to polymixin B,but resistant to Phytomycin.Furthermore,pathogenic test in mice indicated that HLJ-1 isolate was a highly virulent

参考文献/References:

[1] Kielstein P,Rapp-Gabrielson V J. Designation of 15 serovars of Haemophilus parasuis on the basis of immunodiffusion using heat stable antigen extracts. Journal of Clinical Microbiology. 1992
[2] Biberstein E L,White D C. A proposal for the establishment of two new Haemophilus species. Journal of Medical Microbiology. 1969
[3] A Olvera,M Cerda-Cuellar,G Mentaberre,E Casas-Diaz,S Lavin,I Marco,V Aragon. First isolation of Haemophilus parasuis and other NAD-dependent Pasteurellaceae of swine from European wild boars. Veterinary Microbiology. 2007
[4] Simone Oliveira,Carlos Pijoan. Haemophilus parasuis:new trends on di-agnosis,epidemiology and control. Veterinary Microbiology. 2004
[5] Oliveira S,Galina L,Pijoan C. Development of a PCR test to diagnose Haemophilus parasuis infections. . 2001
[6] Zhou Xue-li,,Xu Xiao-juan,Zhao Ya-xin,et al.. Distribution of antimicrobial resistance among different serovars of Haemophilus parasuis isolates[J].. Veterinary Microbiology. 2010
[7] Martín A J,Tuckerb A W,Navas J,et al.. Antimicrobial susceptibility patterns of Haemophilus parasuis from pigs in the United Kingdom and Spain[J].. Veterinary Microbiology. 2001
[8] 蔡旭旺,刘正飞,陈焕春,吴斌,金梅林,Pat Blackall. 副猪嗜血杆菌的分离培养和血清型鉴定[J]. 华中农业大学学报. 2005年01期

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备注/Memo

备注/Memo:
基金:黑龙江省自然基金重点项目(ZJN0503-02)
更新日期/Last Update: 2011-09-29